Anti-15 Lipoxygenase 1抗体(ab80495)
Key features and details
- Goat polyclonal to 15 Lipoxygenase 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-15 Lipoxygenase 1抗体
参阅全部 15 Lipoxygenase 1 一抗 -
描述
山羊多克隆抗体to 15 Lipoxygenase 1 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Orangutan -
免疫原
Synthetic peptide:
C-HYKTDVAVKDDPE
, corresponding to internal sequence amino acids 482-494 of Human 15 Lipoxygenase 1 (NP_001131.3). -
阳性对照
- Nuclear HeLa cell lysate and whole Hela lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab80495 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
说明 |
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WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. -
通路
Lipid metabolism; leukotriene D4 biosynthesis. -
序列相似性
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 246 Human
- Entrez Gene: 100174413 Orangutan
- Omim: 152392 Human
- SwissProt: P16050 Human
- SwissProt: Q5RBE8 Orangutan
- Unigene: 73809 Human
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别名
- 15 lipoxygenase 1 antibody
- 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
- 15 LOX antibody
see all
图片
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Anti-15 Lipoxygenase 1 antibody (ab80495) at 0.2 µg/ml + nuclear HeLa cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (0)
ab80495 尚未被引用在任何文献中。