Anti-14-3-3 sigma/SFN抗体(ab94490)
Key features and details
- Rabbit polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-14-3-3 sigma/SFN抗体
参阅全部 14-3-3 sigma/SFN 一抗 -
描述
兔多克隆抗体to 14-3-3 sigma/SFN -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Cow, Pig -
免疫原
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab105198) -
阳性对照
- This antibody gave a positive signal in Human skin tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94490于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
靶标
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功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
组织特异性
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
细胞定位
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 528453 Cow
- Entrez Gene: 2810 Human
- Entrez Gene: 55948 Mouse
- Entrez Gene: 733625 Pig
- Entrez Gene: 313017 Rat
- Entrez Gene: 443032 Sheep
- Omim: 601290 Human
- SwissProt: Q0VC36 Cow
see all -
别名
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
图片
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Anti-14-3-3 sigma/SFN antibody (ab94490) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94490 尚未被引用在任何文献中。