Anti-14-3-3 gamma/YWHAG抗体(ab155050)
Key features and details
- Rabbit polyclonal to 14-3-3 gamma/YWHAG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-14-3-3 gamma/YWHAG抗体
参阅全部 14-3-3 gamma/YWHAG 一抗 -
描述
兔多克隆抗体to 14-3-3 gamma/YWHAG -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Monkey, Xenopus tropicalis -
免疫原
Recombinant fragment corresponding to Human 14-3-3 gamma/YWHAG aa 1-179.
Database link: P61981 -
阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysate; HeLa cells; Human DLD1 xenograft tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- A-431 whole cell lysate (ab7909)
- MOLT-4 whole cell lysate (ab7912)
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155050于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
组织特异性
Highly expressed in brain, skeletal muscle, and heart. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
Phosphorylated by various PKC isozymes. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 427820 Chicken
- Entrez Gene: 286862 Cow
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Entrez Gene: 56010 Rat
- Entrez Gene: 779762 Xenopus tropicalis
- Omim: 605356 Human
- SwissProt: Q5F3W6 Chicken
see all -
别名
- 14 3 3 gamma antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
图片
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All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : HL-60 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 28 kDa -
All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
12% SDS PAGE -
Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 28 kDa
12% SDS PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma/YWHAG with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma/YWHAG with ab155050 at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab155050 被引用在 4 文献中.
- Ni J et al. Functional genetic variants in centrosome-related genes CEP72 and YWHAG confer susceptibility to gastric cancer. Arch Toxicol 94:2861-2872 (2020). PubMed: 32535685
- Valdebenito S et al. Tunneling Nanotubes Mediate Adaptation of Glioblastoma Cells to Temozolomide and Ionizing Radiation Treatment. iScience 23:101450 (2020). PubMed: 32882515
- Cardona C et al. Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain. Heliyon 5:e01667 (2019). PubMed: 31193135
- Chen XY et al. miR-140-5p mediates bevacizumab-induced cytotoxicity to cardiomyocytes by targeting the VEGFA/14-3-3? signal pathway. Toxicol Res (Camb) 8:875-884 (2019). PubMed: 32190292