10X RIPA缓冲液(ab156034)
概述
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产品名称
10X RIPA缓冲液
参阅全部 Lysis Buffer 试剂 -
经测试应用
适用于: WB, ELISA, SDS-PAGE, IPmore details -
常规说明
Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
Preparation: Dilute to 1X in deionized water
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
性能
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形式
Liquid -
存放说明
Shipped at Room Temperature. Store at Room Temperature. -
存储溶液
pH: 7.50
Constituents: 0.22% Beta glycerophosphate, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% Sodium lauryl sulfate, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate, 10% Nonylphenol, ethoxylated -
Concentration information loading...
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研究领域
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Suggested working concentration: 1X
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ELISA |
Use at an assay dependent concentration. Suggested working concentration: 1X
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SDS-PAGE |
Use at an assay dependent concentration. Suggested working concentration: 1X
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IP |
Use at an assay dependent concentration. Suggested working concentration: 1X
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说明 |
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WB
Use at an assay dependent concentration. Suggested working concentration: 1X |
ELISA
Use at an assay dependent concentration. Suggested working concentration: 1X |
SDS-PAGE
Use at an assay dependent concentration. Suggested working concentration: 1X |
IP
Use at an assay dependent concentration. Suggested working concentration: 1X |
图片
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HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.Lane 1: MW marker
Lane 2: Cleared lysateLane 3: Non-soluble
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (67)
ab156034 被引用在 67 文献中.
- Jansens RJJ et al. Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. Cell Rep 40:111107 (2022). WB, IP, ICC ; Pig . PubMed: 35858564
- Xie L et al. The HIF-1a/p53/miRNA-34a/Klotho axis in retinal pigment epithelial cells promotes subretinal fibrosis and exacerbates choroidal neovascularization. J Cell Mol Med 25:1700-1711 (2021). PubMed: 33438362
- Foray C et al. Imaging temozolomide-induced changes in the myeloid glioma microenvironment. Theranostics 11:2020-2033 (2021). PubMed: 33500706
- Shati AA & El-Kott AF Acylated ghrelin protects against doxorubicin-induced nephropathy by activating silent information regulator 1. Basic Clin Pharmacol Toxicol 128:805-821 (2021). PubMed: 33547742
- Chen Q et al. Rho-kinase inhibitor hydroxyfasudil protects against HIV-1 Tat-induced dysfunction of tight junction and neprilysin/Aß transfer receptor expression in mouse brain microvessels. Mol Cell Biochem 476:2159-2170 (2021). PubMed: 33548010