10x EDTA缓冲液pH 8.0 (ab64216)
概述
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产品名称
10x EDTA缓冲液pH 8.0 -
经测试应用
适用于: IHC-Pmore details -
常规说明
EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).
Recommended Procedure: Microwave
- Deparaffinize and rehydrate tissue sections.
- Dilute the product 1:10 with deionized water.
- Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
- Keep the slides warm by heating for 10min at LOW power.
- Let coplin jar sit in microwave for at least 20min.
- Remove and rinse with buffer.
- Proceed with staining.
性能
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形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
pH: 7.7
Constituent: 0.29% EDTA
Buffer 10x concentrated. -
Concentration information loading...
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研究领域
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相关性
EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies. -
别名
- Ethylenediaminetetraacetic acid buffer
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64216于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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说明 |
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IHC-P
Use at an assay dependent dilution. |
图片
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ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (ab64211)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab64216 被引用在 2 文献中.
- Posta N et al. Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction. Lab Invest 100:986-1002 (2020). PubMed: 32054994
- Hoang JD et al. Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y. JCI Insight 5:N/A (2020). PubMed: 32493842