概述

  • 产品名称
    Anti-Zyxin抗体[ZOL301]
    参阅全部 Zyxin 一抗
  • 描述
    小鼠单克隆抗体[ZOL301] to Zyxin
  • 经测试应用
    适用于: WB, ELISA, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide:

    PPQPREKVSSIDLEIDS

    conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 134-150 of Human Zyxin

  • 阳性对照
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab50391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 61 kDa.
ELISA Use at an assay dependent concentration.

Array: Use at an assay dependent dilution.

ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • 细胞定位
    Cytoplasmic, cytoskeletal and Nuclear.
  • 数据库链接
  • 别名
    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

图片

  • ab50391 staining rat neuronal progenitor cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 4 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.

    See Abreview

文献

This product has been referenced in:
  • Han X  et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »
  • Lock FE  et al. Differential regulation of adhesion complex turnover by ROCK1 and ROCK2. PLoS One 7:e31423 (2012). ICC/IF ; Human . Read more (PubMed: 22348083) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuronal progenitor cell)
Specification
neuronal progenitor cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X100 for 5 minutes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Anne Chiaramello

Verified customer

提交于 Apr 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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