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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab50391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 61 kDa.|
|ELISA||Use at an assay dependent concentration.
Array: Use at an assay dependent dilution.
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab50391 staining rat neuronal progenitor cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 4 µg/ml. An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"