Recombinant fragment: DATGVNADIT ITDIISGLGS NKWIQQNLQC LVLNSLTLSL EDPYERCFSR LSGLRALSIT NVLFYNEDLA EVASLPRLES LDISNTSI, corresponding to amino acids 111-198 of Human ZYG11B Isoform 1 (Q9C0D3).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC.
Belongs to the zyg-11 family.
Contains 2 ARM repeats.
Contains 3 LRR (leucine-rich) repeats.
Information by UniProt
- KIAA1730 antibody
- Protein zyg-11 homolog B antibody
- ZY11B_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZYG11B antibody (ab121975)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab121975 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZYG11B antibody (ab121975)
ab121975 staining ZIG11B in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.
has not yet been referenced specifically in any publications.
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