概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZYG11B
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: DATGVNADIT ITDIISGLGS NKWIQQNLQC LVLNSLTLSL EDPYERCFSR LSGLRALSIT NVLFYNEDLA EVASLPRLES LDISNTSI, corresponding to amino acids 111-198 of Human ZYG11B Isoform 1 (Q9C0D3).

  • 阳性对照
    • Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能
    Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC.
  • 序列相似性
    Belongs to the zyg-11 family.
    Contains 2 ARM repeats.
    Contains 3 LRR (leucine-rich) repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1730 antibody
    • Protein zyg-11 homolog B antibody
    • ZY11B_HUMAN antibody
    • zyg-11 family member B, cell cycle regulator antibody
    • ZYG11 antibody
    • Zyg11b antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab121975 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121975 staining ZIG11B in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

文献

ab121975 has not yet been referenced specifically in any publications.

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