Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling ZWINT with ab71982 at at 1/200 (1µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Western blot - ZWINT antibody (ab71982)
All lanes : Anti-ZWINT antibody (ab71982) at 0.04 µg
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Detection of ZWINT by Western Blot of Immunprecipitate.
ab71982 at 1µg/ml staining ZWINT in HeLa whole cell lysate immunoprecipitated using ab71982 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-ZWINT antibody (ab71982)参考文献
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