The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 5 µg/ml.
应用说明Is unsuitable for WB.
功能Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).
HeLa cell lysate immunoprecipitated using ab105117 (Left Lane) at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) for the analysis of ZWILCH expression. Subsequent WB detection was performed using ab105117 at 1 µg/ml (as the sample is enriched by IP). Rightr Lane represents IP with IgG control.
Exposure time: 10 seconds.
Anti-ZWILCH antibody (ab105117)参考文献
has not yet been referenced specifically in any publications.