Anti-ZW10抗体[3363C4a] (ab53676)
Key features and details
- Mouse monoclonal [3363C4a] to ZW10
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-ZW10抗体[3363C4a]
参阅全部 ZW10 一抗 -
描述
小鼠单克隆抗体[3363C4a] to ZW10 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
ZW10 recombinant fragment, internal sequence, (Human).
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常规说明
The antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3363C4a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53676于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
靶标
- Information by UniProt
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别名
- Centromere/kinetochore protein zw10 antibody
- Centromere/kinetochore protein zw10 homolog antibody
- HZW 10 antibody
see all
图片
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Anti-ZW10 antibody [3363C4a] (ab53676) + Immunizing recombinant ZW10 fragment
Predicted band size: 89 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
数据表及文件
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Datasheet download
文献 (3)
ab53676 被引用在 3 文献中.
- Conti D et al. Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation. Elife 8:N/A (2019). PubMed: 31808746
- Brownlow N et al. Mitotic catenation is monitored and resolved by a PKCe-regulated pathway. Nat Commun 5:5685 (2014). IF . PubMed: 25483024
- Ryu H & Azuma Y Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation. J Biol Chem 285:32576-85 (2010). PubMed: 20696768