Anti-ZSCAN4抗体(ab97748)

概述

  • 产品名称Anti-ZSCAN4抗体
    参阅全部 ZSCAN4 一抗
  • 描述
    兔多克隆抗体to ZSCAN4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ZSCAN4.

    (Peptide available as ab109888.)

  • 阳性对照
    • This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
  • 序列相似性Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • 细胞定位Nucleus. Chromosome > telomere.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ35105 antibody
    • MGC126787 antibody
    • MGC126789 antibody
    • Zinc finger and SCAN domain containing 4 antibody
    • Zinc finger and SCAN domain containing protein 4 antibody
    • Zinc finger and SCAN domain-containing protein 4 antibody
    • Zinc finger protein 494 antibody
    • ZNF 494 antibody
    • ZNF494 antibody
    • ZSCA4_HUMAN antibody
    • ZSCAN4 antibody
    see all

Anti-ZSCAN4 antibody 图像

  • All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-ZSCAN4 antibody (ab97748)参考文献

ab97748 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SHEF3 hESC)
Loading amount 20 µg
Specification SHEF3 hESC
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 08 2012

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