Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ZSCAN4.
(Peptide available as
This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).
Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
Contains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain.
Nucleus. Chromosome > telomere.
Information by UniProt
Western blot - ZSCAN4 antibody (ab97748)
All lanes :
Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml
Lane 1 :
Human kidney tissue lysate - total protein (
Lane 2 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 :
A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 :
HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
58 kDa (
why is the actual band size different from the predicted?
Additional bands at :
38 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"