antigen, corresponding to amino acids 111-177 (HRPVSGEEAV TVLEDLEREL DDPGEQVLSH AHEQEEFVKE KATPGAAQES SNDQFQTLEE QLGYNLR) of Human ZSCAN23.
Human smooth muscle tissue
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Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. Contains 1 SCAN box domain.
Information by UniProt
Zinc finger and SCAN domain-containing protein 23 antibody
Zinc finger protein 390 antibody
Zinc finger protein 453 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZSCAN23 antibody (ab122303)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122303 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZSCAN23 antibody (ab122303)
ab122303, at 1/450, staining ZSCAN23 in paraffin embedded Human smooth muscle tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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