Anti-ZP3抗体(ab70384)
Key features and details
- Rabbit polyclonal to ZP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZP3抗体 -
描述
兔多克隆抗体to ZP3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ZP3 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87447) -
阳性对照
- This antibody gave a positive signal in JEG-3 Whole Cell Lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70384于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. -
组织特异性
Oocytes. -
序列相似性
Belongs to the ZP domain family. ZPC subfamily.
Contains 1 ZP domain. -
结构域
The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida. -
翻译后修饰
Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy. -
细胞定位
Secreted > extracellular space > extracellular matrix and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7784 Human
- Omim: 182889 Human
- SwissProt: P21754 Human
- Unigene: 656137 Human
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别名
- HUMZP3 antibody
- Processed zona pellucida sperm-binding protein 3 antibody
- Sperm receptor antibody
see all
图片
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Anti-ZP3 antibody (ab70384) at 1 µg/ml + JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 17 kDa (possible cleavage fragment)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab70384 被引用在 1 文献中.
- Du D et al. Senotherapy Protects against Cisplatin-Induced Ovarian Injury by Removing Senescent Cells and Alleviating DNA Damage. Oxid Med Cell Longev 2022:9144644 (2022). PubMed: 35693700