概述

  • 产品名称Anti-ZP3抗体
    参阅全部 ZP3 一抗
  • 描述
    兔多克隆抗体to ZP3
  • 经测试应用适用于: ICC, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    A region within synthetic peptide: QWSRSASRNR RHVTEEADVT VGPLIFLDRR GDHEVEQWAL PSDTSVVLLG, corresponding to internal sequence amino acids 342-391 of Human ZP3

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab48895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
  • 组织特异性Oocytes.
  • 序列相似性Belongs to the ZP domain family. ZPC subfamily.
    Contains 1 ZP domain.
  • 结构域The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • 翻译后修饰Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    N-glycosylated.
    O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.
  • 细胞定位Secreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HUMZP3 antibody
    • Processed zona pellucida sperm-binding protein 3 antibody
    • Sperm receptor antibody
    • zona pellucida glycoprotein 3 (sperm receptor) antibody
    • Zona pellucida glycoprotein 3 antibody
    • zona pellucida glycoprotein 3A (sperm receptor) antibody
    • Zona pellucida glycoprotein ZP3 antibody
    • Zona pellucida protein C antibody
    • Zona pellucida sperm binding protein 3 precursor antibody
    • Zp-3 antibody
    • ZP3 372 antibody
    • ZP3 424 antibody
    • ZP3 474 antibody
    • ZP3 antibody
    • ZP3_HUMAN antibody
    • ZP3A antibody
    • ZP3A/ZP3B antibody
    • ZP3B antibody
    • ZPC antibody
    see all

Anti-ZP3 antibody 图像

  • Anti-ZP3 antibody (ab48895) at 0.6 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-ZP3 antibody (ab48895)参考文献

ab48895 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Mouse Cell (Whole mouse embryo)
Specification Whole mouse embryo
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 21 2013

Application Immunocytochemistry
Sample Mouse Cultured Cells (Embryonic Stem Cell - R1 line)
Specification Embryonic Stem Cell - R1 line
Fixative Paraformaldehyde
Permeabilization Yes - Cold 0.5% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Rebekah Gundry

Verified customer

提交于 Oct 07 2010

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cell (R1 & D3 cell lines))
Loading amount 50 µg
Specification Mouse embryonic stem cell (R1 & D3 cell lines)
Gel Running Conditions Reduced Denaturing (4-12% NuPage Bis-Tris gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"