Anti-ZP1抗体(ab90480)

概述

  • 产品名称Anti-ZP1抗体
    参阅全部 ZP1 一抗
  • 描述
    兔多克隆抗体to ZP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide designed within residues: VGFEDSYGQE PTLGPTDSNG NSSLRPLLWA VLLLPAVALV LGFGVFVGLS, corresponding to internal amino acids 576 - 625 of Human ZP1 (NP_997224).

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab90480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
  • 组织特异性Oocytes.
  • 序列相似性Belongs to the ZP domain family. ZPB subfamily.
    Contains 1 P-type (trefoil) domain.
    Contains 1 ZP domain.
  • 结构域The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • 翻译后修饰Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    O-glycosylated.
  • 细胞定位Secreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC87693 antibody
    • Processed zona pellucida sperm-binding protein 1 antibody
    • zona pellucida glycoprotein 1 (sperm receptor) antibody
    • Zona pellucida glycoprotein 1 antibody
    • zona pellucida protein 1 antibody
    • Zp 1 antibody
    • Zp-1 antibody
    • Zp1 antibody
    • ZP1_HUMAN antibody
    see all

Anti-ZP1 antibody 图像

  • Anti-ZP1 antibody (ab90480) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa

Anti-ZP1 antibody (ab90480)参考文献

ab90480 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting my colleague at Abcam. To our knowledge, no one has tested this for ICC/IF. Trying a different fixative, for instance methanol instead of paraformaldehyde, may give a better result, but we have not tried either.

I r...

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