The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/100 - 1/5000.
WB: 1/100 - 1/1000. Detects a band of approximately 45 kDa.
The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Zona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
Coating: Plasma (diluted 1:1000) from 17ß-estradiol treated (E2) or control (C) fish.
Western blot - zona radiata antibody [MN-7F2] (ab36802)
Lane 1 : Molecular weight standards Lanes 2-10 : Anti-zona radiata antibody [MN-7F2] (ab36802) at 1/500 dilution
Lane 2 : Purified Zrp, [alpha, beta and gamma]. Lane 3 : Plasma from 17ß-estradiol treated Salmon at 1/200 dilution Lane 4 : Plasma from control Salmon at 1/200 dilution Lane 5 : Plasma from 17ß-estradiol treated Arctic char at 1/200 dilution Lane 6 : Plasma from control Arctic char at 1/200 dilution Lane 7 : Plasma from 17ß-estradiol treated Turbot at 1/200 dilution Lane 8 : Plasma from control Turbot at 1/200 dilution Lane 9 : Plasma from 17ß-estradiol treated Cod at 1/200 dilution Lane 10 : Plasma from control Cod at 1/200 dilution