Anti-zona radiata抗体[MN-7F2] (ab36802)

概述

  • 产品名称Anti-zona radiata抗体[MN-7F2]
    参阅全部 zona radiata 一抗
  • 描述
    小鼠单克隆抗体[MN-7F2] to zona radiata
  • 特异性This antibody binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the g monomer.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Rainbow Trout
  • 免疫原

    Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).

  • 阳性对照
    • Plasma from 17ß-estradiol treated Salmon.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Affinity purified
  • 克隆单克隆
  • 克隆编号MN-7F2
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab36802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/100 - 1/5000.
    WB: 1/100 - 1/1000. Detects a band of approximately 45 kDa.
    The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Zona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
    • 数据库链接
      • 别名
        • ZRP antibody

      Anti-zona radiata antibody [MN-7F2] 图像

      • Coating: Plasma (diluted 1:1000) from 17ß-estradiol treated (E2) or control (C) fish.
      • Lane 1 : Molecular weight standards
        Lanes 2 - 10 : Anti-zona radiata antibody [MN-7F2] (ab36802) at 1/500 dilution

        Lane 1 : As above
        Lane 2 : Purified Zrp, [alpha, beta and gamma].
        Lane 3 : Plasma from 17ß-estradiol treated Salmon at 1/200 dilution
        Lane 4 : Plasma from control Salmon at 1/200 dilution
        Lane 5 : Plasma from 17ß-estradiol treated Arctic char at 1/200 dilution
        Lane 6 : Plasma from control Arctic char at 1/200 dilution
        Lane 7 : Plasma from 17ß-estradiol treated Turbot at 1/200 dilution
        Lane 8 : Plasma from control Turbot at 1/200 dilution
        Lane 9 : Plasma from 17ß-estradiol treated Cod at 1/200 dilution
        Lane 10 : Plasma from control Cod at 1/200 dilution

      Anti-zona radiata antibody [MN-7F2] (ab36802)参考文献

      ab36802 has not yet been referenced specifically in any publications.

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