Anti-ZO1 tight junction protein抗体(ab99462)


  • 产品名称Anti-ZO1 tight junction protein抗体
    参阅全部 ZO1 tight junction protein 一抗
  • 描述
    山羊多克隆抗体to ZO1 tight junction protein
  • 特异性ab99462 is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Peptide with sequence


    , from the internal region of the protein sequence according to NP_003248.3; NP_783297.2.

  • 阳性对照
    • Human brain (cerebral cortex) lysate


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab99462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 195 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24874215


  • 功能The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • 组织特异性The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • 结构域The second PDZ domain mediates interaction with GJA12.
  • 翻译后修饰Phosphorylated. Dephosphorylated by PTPRJ.
  • 细胞定位Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Anti-ZO1 tight junction protein antibody 图像

  • Anti-ZO1 tight junction protein antibody (ab99462) at 0.3 µg/ml + Human brain (cerebral cortex) lysate at 35 µg
    developed using the ECL technique

    Predicted band size : 195 kDa

    Exposure time : 1 hour

Anti-ZO1 tight junction protein antibody (ab99462)参考文献

This product has been referenced in:
  • Lundin A  et al. Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus. PLoS Pathog 10:e1004166 (2014). ICC/IF ; Human . Read more (PubMed: 24874215) »

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