Anti-ZO1 tight junction protein抗体(ab84934)


  • 产品名称Anti-ZO1 tight junction protein抗体
    参阅全部 ZO1 tight junction protein 一抗
  • 描述
    兔多克隆抗体to ZO1 tight junction protein
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human ZO1 tight junction protein.

    (Peptide available as ab101198.)

  • 阳性对照
    • This antibody gave a positive signal in Human Lung Tissue Lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab84934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 195 kDa).


  • 功能The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • 组织特异性The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • 结构域The second PDZ domain mediates interaction with GJA12.
  • 翻译后修饰Phosphorylated. Dephosphorylated by PTPRJ.
  • 细胞定位Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Anti-ZO1 tight junction protein antibody 图像

  • Anti-ZO1 tight junction protein antibody (ab84934) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 195 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-ZO1 tight junction protein antibody (ab84934)参考文献

ab84934 has not yet been referenced specifically in any publications.

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