为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein
ab59720 recognises both ZO1 tight junction alpha-minus and alpha-plus isoforms.
The protein is abundant in endothelial cells and the highly specialized epithelial junctions.
Customers report variable results in IHC-Fr and IHC-P application; though we have nice image for IHC-Fr. If you would like to use ab59720 in IHC-Fr then we suggest using ethanol for fixation instead of acetone.
IHC-Fr and IHC-P are therefore not covered by Abpromise guarantee.
The immunogen sequence for this product has 92.5% homology with the mouse sequence however in our hands we have not been able to produce positive data with mouse samples.
We have also received mixed feedback for Pig cross reactivity so we no longer guarantee this species. One of the reviewer have successfully used this antibody with pig samples so we are keen to receive more feedback from other customers.
Our Abpromise guarantee covers the use of ab59720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.
Fix cells with 4% paraformaldehyde and permeabilize with PBS + 0.1% Saponin (+ 0.5% BSA).
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/50 - 1/250. Predicted molecular weight: 194 kDa.
Abcam recommends BSA blocking.
Immunofluorescence staining in Huvec cells.
Flow cytometry with Huvec cells. The black line represents the cells only, the red line the control and the blue line ab59720 at 10 μg/ml.
ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"