功能May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
组织特异性Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
通路Protein modification; protein ubiquitination.
序列相似性Contains 1 RING-type zinc finger.
结构域The RING-type zinc finger domain is required for E3 ligase activity.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Endosome membrane. Lysosome membrane. Cell junction > synapse > presynaptic cell membrane. Present in presynaptic plasma membranes in neurons.
All lanes : Anti-ZNRF2 antibody (ab86778) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 24 kDa
Exposure time : 3 minutes
Immunoprecipitation - ZNRF2 antibody (ab86778)
HeLa whole cell lysate (1 mg) was immunoprecipitated with 10 µg ab86778 (lane 1) or control IgG antibody (lane 2). 20% of the immunoprecipitate was subjected to Western blotting and detected with ab86778 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 30 seconds.
Anti-ZNRF2 antibody (ab86778)参考文献
has not yet been referenced specifically in any publications.