The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 10 µg/mg of lysate.
Is unsuitable for WB.
May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
Protein modification; protein ubiquitination.
Contains 1 RING-type zinc finger.
The RING-type zinc finger domain is required for E3 ligase activity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Endosome membrane. Lysosome membrane. Cell junction > synapse > presynaptic cell membrane. Present in presynaptic plasma membranes in neurons.
Rabbit anti-ZNRF2 antibody, at 1 µg/ml, staining ZNRF2 in HeLa whole cell lysate immunoprecipitated using ab114098 at 10 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.