Anti-ZNHIT2抗体(ab118133)

概述

  • 产品名称Anti-ZNHIT2抗体
    参阅全部 ZNHIT2 一抗
  • 描述
    小鼠单克隆抗体to ZNHIT2
  • 经测试应用适用于: ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 274-362 of Human ZNHIT2 with proprietary tag (NP_055020).

性能

应用

Our Abpromise guarantee covers the use of ab118133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

靶标

  • 组织特异性Low expression in most tissues; highly expressed in testis.
  • 序列相似性Contains 1 HIT-type zinc finger.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C11orf5 antibody
    • FON antibody
    • MGC120285 antibody
    • MGC120286 antibody
    • OTTHUMP00000230419 antibody
    • Protein FON antibody
    • Zinc finger HIT domain-containing protein 2 antibody
    • Zinc finger HIT type containing 2 antibody
    • zinc finger, HIT domain containing 2 antibody
    • zinc finger, HIT type 2 antibody
    • ZNHI2_HUMAN antibody
    • ZNHIT2 antibody
    see all

Anti-ZNHIT2 antibody 图像

  • Detection limit for recombinant tagged ZNHIT2 is 1 ng/ml as a capture antibody.

Anti-ZNHIT2 antibody (ab118133)参考文献

ab118133 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview for ab118133. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested ssamples and application, we would like to follo...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa S3)
Loading amount 5.6 µg
Specification HeLa S3
Treatment two succesive rounds of siRNA (100 nM). One directed agaist ZNHIT2 and three controls
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Aug 30 2012

Thank you for the image. I encourage you to include the image in your review. I agree it does not look very good but this is useful data, insofar as it shows what does not work.

The discount code will be active as soon as you submit your rev...

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I am very pleased to hear you would like to accept our offer and test ab118133 in western blotting. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for western blotting and i...

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Thank you for your reply, yes, we would be interested in adding western blot data. As you can see from the datasheet, the antibody is not very well-characterized, and specificity has not been tested on endogenous samples of any kind. What kind of sampl...

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