概述

  • 产品名称
    Anti-ZNF826抗体
  • 描述
    兔多克隆抗体to ZNF826
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 109-158 HKHRRTHTGEKPYKCEECGKAFTASSTLSEYKTIHTGEKPCKCEECGKAF of Human ZNF826, NP_001034973

  • 阳性对照
    • fetal liver lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 相关性
    May be involved in transcriptional regulation.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • FLJ44894 antibody
    • Zinc finger protein 826 antibody

图片

  • Anti-ZNF826 antibody (ab81375) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : <22 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • González-Prieto R  et al. c-Myc is targeted to the proteasome for degradation in a SUMOylation-dependent manner, regulated by PIAS1, SENP7 and RNF4. Cell Cycle 14:1859-72 (2015). Read more (PubMed: 25895136) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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