产品名称 Anti-ZNF8抗体 参阅全部 ZNF8 一抗
WB, IP more details
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 525 and 575 of Human ZNF8 (NP_066575.1).
HeLa and 293T whole cell lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
Use at 5-10 µg/mg of lysate.
功能 May be involved in transcriptional regulation.
组织特异性 Ubiquitously present in many human cell lines of different embryological derivation.
序列相似性 Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers. Contains 1 KRAB domain.
翻译后修饰 Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Zinc finger protein 272 antibody
Anti-ZNF8 antibody 图像
Western blot - ZNF8 antibody (ab87732)
All lanes : Anti-ZNF8 antibody (ab87732) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 65 kDa Observed band size : 70 kDa ( why is the actual band size different from the predicted?) Exposure time : 3 minutes
Immunoprecipitation - ZNF8 antibody (ab87732)
Detection of ZNF8 in Immunoprecipitates of Hela whole cell lysate (1 mg for IP, 20% of IP loaded) using ab87732 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Anti-ZNF8 antibody (ab87732)参考文献
has not yet been referenced specifically in any publications.
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