, corresponding to amino acids 1-72 of Human ZNF774
Human colon tissue; RT4 cell lysate and Human Plasma
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 12 C2H2-type zinc fingers. Contains 1 KRAB domain.
Information by UniProt
Zinc finger protein 774 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZNF774 antibody (ab122137)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and cytoskeleton (intermediate filaments). Recommended concentration of ab122137 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF774 antibody (ab122137)
ab122137, at 1/200 dilution, staining ZNF774 in Human colon tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Western blot - Anti-ZNF774 antibody (ab122137)
All lanes : Anti-ZNF774 antibody (ab122137) at 1/500 dilution Lane 1 : RT4 cell lysate Lane 2 : U251MG cell lysate Lane 3 : Human Plasma Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate
has not yet been referenced specifically in any publications.
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