, corresponding to amino acids 274-303 of Human ZNF749
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 19 C2H2-type zinc fingers. Contains 1 KRAB domain.
Information by UniProt
Zinc finger protein 749 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZNF749 antibody (ab122771)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and nuclear membrane. Recommended concentration of ab122771 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF749 antibody (ab122771)
ab122771, at 1/200 dilution, staining ZNF749 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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