antigen sequence AQASLLACEG LAGVSLVPTA ASKKMMLSQI ASKQAENGER AGSPDVLRCS SQGHRKDSDK SRSRKDD, corresponding to N terminal amino acids 2-68 of Human ZNF740 (UniProt ID: Q8NDX6).
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Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Information by UniProt
OriLyt TD-element-binding protein 7 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZNF740 antibody (ab122290)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab122290 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF740 antibody (ab122290)
ab122290, at a 1/50 dilution, staining ZNF740 in paraffin embedded Human urinary bladder tissue by Immunohistochemistry.
Western blot - Anti-ZNF740 antibody (ab122290)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
has not yet been referenced specifically in any publications.
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