Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human ZNF644 (NP_958357.1).
Jurkat, HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab117777 was affinity purified using an epitope specific to ZNF644 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 150 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Information by UniProt
Western blot - Anti-ZNF644 antibody (ab117777)
All lanes : Anti-ZNF644 antibody (ab117777) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 150 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-ZNF644 antibody (ab117777)
Detection of ZNF644 by Western Blot of Immunprecipitate.
ab117777 at 1µg/ml staining ZNF644 in 293T whole cell lysate immunoprecipitated using ab117777 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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