概述

  • 产品名称Anti-ZNF621抗体
    参阅全部 ZNF621 一抗
  • 描述
    兔多克隆抗体to ZNF621
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide: LSSNTALTQH QRIHTGEKPY ECKECGKAFR RSAAYLQHQR LHTGEKLYKC, corresponding to a region within internal sequence amino acids 216-265 of Human ZNF621

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab49225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

靶标

Anti-ZNF621 antibody 图像

  • Anti-ZNF621 antibody (ab49225) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 49 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-ZNF621 antibody (ab49225)参考文献

ab49225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"