概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF615
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment from a region of Human ZNF615.

  • 阳性对照
    • Human appendix tissue and Human cell line A-431.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

图片

  • Immunohistochemical staining of paraffin embeddedHhuman appendix tissue with anti-ZNF615 antibody (ab121140; dilution 1:100) showing strong cytoplasmic positivity in lymphoid tissue.
  • Immunofluorescent staining of human cell line A-431 with anti-ZNF615 antibody (ab121140) at 4 µg/ml showing positivity in nucleus but not nucleoli.

文献

ab121140 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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