概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF583
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from a region within N terminal amino acids 72-121 (TRGPCPDWEY VFKNSEFSSK QETYEESSKV VTVGARHLSY SLDYPSLRED) of Human ZNF583 (NP_689691)

  • 阳性对照
    • Jurkat cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May be involved in transcriptional regulation.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 12 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ31030 antibody
    • MGC133237 antibody
    • Zinc finger protein 583 antibody
    • Zinc finger protein L3-5 antibody
    • ZN583_HUMAN antibody
    • ZNF583 antibody
    see all

Anti-ZNF583 antibody 图像

  • Anti-ZNF583 antibody (ab85635) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-ZNF583 antibody (ab85635)参考文献

ab85635 has not yet been referenced specifically in any publications.

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