参阅全部 ZNF577 一抗
WB, ELISAmore details
A region within synthetic peptide: KAFAHMSVLI KHEKTHIRET AINSLTVEKP SSRSHTSLYM SELIQEQKTV, corresponding to C terminal amino acids 361-410 of Human ZNF577
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using a peptide based assay was 1/1562500.
- 相关性ZNF577 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 8 C2H2-type zinc fingers and 1 KRAB domain. It is thought to be involved in transcriptional regulation.
- 细胞定位Nuclear (probable)
- FLJ32291 antibody
- MGC 4400 antibody
- MGC4400 antibody
Anti-ZNF577 antibody 图像
Western blot - ZNF577 antibody (ab66381)
Anti-ZNF577 antibody (ab66381) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 54 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa,36 kDa. We are unsure as to the identity of these extra bands.
Anti-ZNF577 antibody (ab66381)参考文献
has not yet been referenced specifically in any publications.
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