概述

  • 产品名称Anti-ZNF574抗体
  • 描述
    兔多克隆抗体to ZNF574
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 846 and 896 of human ZNF574 (NP_073589.4).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab85933 was affinity purified using an epitope specific to ZNF574 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab85933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 99 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-ZNF574 antibody 图像

    • All lanes : Anti-ZNF574 antibody (ab85933) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 99 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of human ZNF54 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85933 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-ZNF574 antibody (ab85933)参考文献

    ab85933 has not yet been referenced specifically in any publications.

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