Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to a region between residues 846 and 896 of human ZNF574 (NP_073589.4).
- HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab85933 was affinity purified using an epitope specific to ZNF574 immobilized on solid support.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 99 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 20 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- FLJ22059 antibody
- FP972 antibody
- Zinc finger protein 574 antibody
Western blot - ZNF574 antibody (ab85933)
All lanes :
Anti-ZNF574 antibody (ab85933) at 0.4 µg/mlLane 1 :
HeLa whole cell lysate at 50 µgLane 2 :
HeLa whole cell lysate at 15 µgLane 3 :
HeLa whole cell lysate at 5 µgLane 4 :
293T whole cell lysate at 50 µgPredicted band size:
99 kDaObserved band size:
120 kDa (why is the actual band size different from the predicted?
developed using the ECL technique
Immunoprecipitation - ZNF574 antibody (ab85933)
Detection of human ZNF54 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85933 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"