Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to a region between residues 846 and 896 of human ZNF574 (NP_073589.4).
- HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab85933 was affinity purified using an epitope specific to ZNF574 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 99 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 20 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- FLJ22059 antibody
- FP972 antibody
- Zinc finger protein 574 antibody
Western blot - Anti-ZNF574 antibody (ab85933)
All lanes :
Anti-ZNF574 antibody (ab85933) at 0.4 µg/mlLane 1 :
HeLa whole cell lysate at 50 µgLane 2 :
HeLa whole cell lysate at 15 µgLane 3 :
HeLa whole cell lysate at 5 µgLane 4 :
293T whole cell lysate at 50 µgPredicted band size:
99 kDaObserved band size:
120 kDa (why is the actual band size different from the predicted?
developed using the ECL technique
Immunoprecipitation - Anti-ZNF574 antibody (ab85933)
Detection of human ZNF54 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85933 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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