概述

  • 产品名称Anti-ZNF543抗体
    参阅全部 ZNF543 一抗
  • 描述
    兔多克隆抗体to ZNF543
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: GCPLFKPELI YQLDHRQELW MATKDLSQSS YPGDNTKPKT TEPTFSHLAL PEEVLLQEQL TQGASKNSQL GQSK, corresponding to amino acids in Human ZNF543.

  • 阳性对照
    • Human rectum tissue; RT 4, U 251 MG, Human plasma, liver and tonsil lysates.

性能

应用

Our Abpromise guarantee covers the use of ab122080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

Anti-ZNF543 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122080 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122080, at 1/50 dilution, staining ZNF543 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-ZNF543 antibody (ab122080) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

Anti-ZNF543 antibody (ab122080)参考文献

ab122080 has not yet been referenced specifically in any publications.

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