Anti-ZNF524抗体(ab87224)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF524
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (PGGATSSNRT LKASLPRKRG RPPKSGQEPP LVQVQGVTAP VGSSGGSDLL) of Human ZNF524 (NP_694951).

  • 阳性对照
    • MCF7 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-ZNF524 antibody 图像

  • Anti-ZNF524 antibody (ab87224) at 1 µg/ml (5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa

实验方案

Anti-ZNF524 antibody (ab87224)参考文献

ab87224 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (SILAC labeled HeLa S3 nuclear extract)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
SILAC labeled HeLa S3 nuclear extract
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提交于 Apr 06 2016

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