Anti-ZNF516抗体(ab121486)

概述

  • 产品名称
    Anti-ZNF516抗体
  • 描述
    兔多克隆抗体to ZNF516
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 610-714 of Human ZNF516.

  • 阳性对照
    • Human prostate tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May be involved in transcriptional regulation.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 10 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C330029B10Rik antibody
    • D230016L03 antibody
    • HsT287 antibody
    • KIAA0222 antibody
    • RGD1306817 antibody
    • Zfp26l antibody
    • Zinc finger protein 516 antibody
    • ZN516_HUMAN antibody
    • Znf516 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121486 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121486, at 1/60 dilution, staining ZNF516 in paraffin-embedded Human prostate tissue by Immunohistochemistry, showing granular cytoplasmic positivity in glandular cells.

文献

ab121486 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (white adipose tissue, brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
80 µg
Specification
white adipose tissue, brown adipose tissue
Blocking step
Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Aug 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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