Anti-ZNF516抗体(ab121486)
Key features and details
- Rabbit polyclonal to ZNF516
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZNF516抗体 -
描述
兔多克隆抗体to ZNF516 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 610-714 of Human ZNF516.
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阳性对照
- Human prostate tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in transcriptional regulation. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 10 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9658 Human
- SwissProt: Q92618 Human
- Unigene: 436973 Human
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别名
- C330029B10Rik antibody
- D230016L03 antibody
- HsT287 antibody
see all
图片
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121486 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121486, at 1/60 dilution, staining ZNF516 in paraffin-embedded Human prostate tissue by Immunohistochemistry, showing granular cytoplasmic positivity in glandular cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121486 被引用在 2 文献中.
- E J et al. ZNF516 suppresses stem cell-like characteristics by regulating the transcription of Sox2 in colorectal cancer. Am J Cancer Res 12:3745-3759 (2022). PubMed: 36119845
- Ili C et al. Loss of ZNF516 protein expression is related with HR-HPV infection and cervical preneoplastic lesions. Arch Gynecol Obstet N/A:N/A (2018). PubMed: 30564931