概述

  • 产品名称Anti-ZNF498抗体
    参阅全部 ZNF498 一抗
  • 描述
    兔多克隆抗体to ZNF498
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit
  • 免疫原

    Synthetic peptide: DCFGEYVEPQ DCRVSPGGGS KEKEAKPPQE DLKGALVALT SERFGEASLQ, corresponding to amino acids 253-302 of Human ZNF498

  • 阳性对照
    • Jurkat cell lysate, human intestinal tissue.

性能

应用

Our Abpromise guarantee covers the use of ab58700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

靶标

Anti-ZNF498 antibody 图像

  • Anti-ZNF498 antibody (ab58700) at 1.25 µg/ml + Jurkat cell extract at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling ZNF498 with ab58700 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of intestinal villi. Magnification: 400X.

Anti-ZNF498 antibody (ab58700)参考文献

ab58700 has not yet been referenced specifically in any publications.

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