Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Titre using peptide based assay: 1/62500.
||Use a concentration of 0.25 µg/ml. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-ZNF485 antibody 图像
Western blot - ZNF485 antibody (ab50559)
Lane 1 : Lane 2 :
Anti-ZNF485 antibody (ab50559) at 0.25 µg/mlLane 1 :
MARKERLane 2 :
HepG2 cell lysate at 10 µgSecondaryLane 2 :
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
46 kDaObserved band size :
35,50 kDa (why is the actual band size different from the predicted?
Anti-ZNF485 antibody (ab50559)参考文献
has not yet been referenced specifically in any publications.
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