Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 2456-2506 of Human ZNF462 (NP_067047.4).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab117771 was affinity purified using an epitope specific to ZNF462 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 285 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Contains 27 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - Anti-ZNF462 antibody (ab117771)
All lanes : Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Developed using the ECL technique Predicted band size : 285 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-ZNF462 antibody (ab117771)
Detection of ZNF462 by Western Blot of Immunprecipitate.
ab117771 at 1µg/ml staining ZNF462 in 293T whole cell lysate immunoprecipitated using ab117771 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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