Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog
Synthetic peptide: EECGCSFSWK SQLVIHRKSH TGQRRHFCSD CGRAFDWKSQ LVIHRKGHRP, corresponding to a region within C terminal amino acids 398-447 of Human ZNF446
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Contains 1 SCAN box domain.
Information by UniProt
- FLJ20626 antibody
- Zinc finger protein 446 antibody
- Zinc finger protein with KRAB and SCAN domains 20 antibody
Western blot - ZNF446 antibody (ab66657)
Anti-ZNF446 antibody (ab66657) at 1.25 µg/ml + (B) HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
49 kDaObserved band size:
55 kDa (why is the actual band size different from the predicted?
)Additional bands at:
36 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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