概述

  • 产品名称Anti-ZNF444抗体
    参阅全部 ZNF444 一抗
  • 描述
    兔多克隆抗体to ZNF444
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human ZNF444 according to NP_060807.2.

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab117812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Transcriptional regulator. Binds to the 5'-flanking critical region of the SCARF1 promoter.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Endothelial zinc finger protein 2 antibody
    • EZF 2 antibody
    • EZF-2 antibody
    • EZF2 antibody
    • FLJ11137 antibody
    • ZCCAN17 antibody
    • Zinc finger and SCAN domain-containing protein 17 antibody
    • Zinc finger protein 444 antibody
    • ZN444_HUMAN antibody
    • ZNF444 antibody
    • ZSCAN17 antibody
    see all

Anti-ZNF444 antibody 图像

  • All lanes : Anti-ZNF444 antibody (ab117812) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 35 kDa


    Exposure time : 30 seconds
  • ab117812 at 1 µg/ml staining ZNF444 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab117812 at 6 µg/mg lysate.
    Lane 2; IP using an antibody to a downstream epitope.
    Lane 3; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.

Anti-ZNF444 antibody (ab117812)参考文献

ab117812 has not yet been referenced specifically in any publications.

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