Anti-ZNF426抗体(ab30967)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF426
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A region within synthetic peptide: VMLENYKNLA TVGGQIIKPS LISWLEQEES RTVQGGVLQG WEMRLETQWS, corresponding to N terminal amino acids 70-119 of Human ZNF426

  • 阳性对照
    • Fetal liver lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab30967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-ZNF426 antibody (ab30967) at 0.5 µg/ml (in 5% skim milk / PBS) + Fetal liver lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab30967 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Multiple cell types)
Loading amount
30 µg
Specification
Multiple cell types
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Seth Frietze

Verified customer

提交于 Jul 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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