• 产品名称Anti-ZNF423抗体
    参阅全部 ZNF423 一抗
  • 描述
    兔多克隆抗体to ZNF423
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 1235 - 1284 (FTVFVQANKL QQHIFAVHGQ EDKIYDCSQC PQKFFFQTEL QNHTMSQHAQ) of Human ZNF423 (NP_055884).

  • 阳性对照
    • HT1080 cell lysate


Our Abpromise guarantee covers the use of ab94451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 功能Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
  • 组织特异性Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 30 C2H2-type zinc fingers.
  • 结构域Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Early B cell factor associated zinc finger protein antibody
    • Ebfaz antibody
    • hOAZ antibody
    • JBTS19 antibody
    • KIAA0760 antibody
    • NPHP14 antibody
    • Nur12 antibody
    • OAZ antibody
    • OLF 1/EBF associated zinc finger antibody
    • OLF1/EBF associated zinc finger protein antibody
    • Olf1/EBF-associated zinc finger protein antibody
    • Roaz antibody
    • Smad and Olf interacting zinc finger protein antibody
    • Smad- and Olf-interacting zinc finger protein antibody
    • Zfp104 antibody
    • ZFP423 antibody
    • zinc finger protein 423, mouse, homolog of antibody
    • Zinc finger protein 423 antibody
    • ZN423_HUMAN antibody
    • ZNF423 antibody
    • ZNF423 zinc finger protein 423 antibody
    see all

Anti-ZNF423 antibody 图像

  • Anti-ZNF423 antibody (ab94451) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 145 kDa

Anti-ZNF423 antibody (ab94451)参考文献

ab94451 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (fibrosarcoma cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification fibrosarcoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Aug 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293FT overexpression FUW-ZNF423)
Specification 293FT overexpression FUW-ZNF423
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X100 and 0.1% TW20 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 25 2012