概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF423
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 1235 - 1284 (FTVFVQANKL QQHIFAVHGQ EDKIYDCSQC PQKFFFQTEL QNHTMSQHAQ) of Human ZNF423 (NP_055884).

  • 阳性对照
    • HT1080 cell lysate

应用

Our Abpromise guarantee covers the use of ab94451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
  • 组织特异性
    Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 30 C2H2-type zinc fingers.
  • 结构域
    Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Early B cell factor associated zinc finger protein antibody
    • Ebfaz antibody
    • hOAZ antibody
    • JBTS19 antibody
    • KIAA0760 antibody
    • NPHP14 antibody
    • Nur12 antibody
    • OAZ antibody
    • OLF 1/EBF associated zinc finger antibody
    • OLF1/EBF associated zinc finger protein antibody
    • Olf1/EBF-associated zinc finger protein antibody
    • Roaz antibody
    • Smad and Olf interacting zinc finger protein antibody
    • Smad- and Olf-interacting zinc finger protein antibody
    • Zfp104 antibody
    • ZFP423 antibody
    • zinc finger protein 423, mouse, homolog of antibody
    • Zinc finger protein 423 antibody
    • ZN423_HUMAN antibody
    • ZNF423 antibody
    • ZNF423 zinc finger protein 423 antibody
    see all

图片

  • Anti-ZNF423 antibody (ab94451) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 145 kDa

文献

This product has been referenced in:
  • Chung GT  et al. Identification of a recurrent transforming UBR5-ZNF423 fusion gene in EBV-associated nasopharyngeal carcinoma. J Pathol 231:158-67 (2013). WB . Read more (PubMed: 23878065) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (fibrosarcoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
fibrosarcoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293FT overexpression FUW-ZNF423)
Specification
293FT overexpression FUW-ZNF423
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X100 and 0.1% TW20 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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