Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
A region within synthetic peptide: RFRSLSSLRA HLEFSHSYEE RTLLTKCSLF PSLKDTDLVT SSELLKPGKL, corresponding to N terminal amino acids 36-85 of Human ZNF365
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
||Use a concentration of 4 - 8 µg/ml.
Anti-ZNF365 antibody 图像
Western blot - ZNF365 antibody (ab49243)
Anti-ZNF365 antibody (ab49243) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
38 kDaObserved band size :
41 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ZNF365 antibody(ab49243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ZNF365 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.
Anti-ZNF365 antibody (ab49243)参考文献
has not yet been referenced specifically in any publications.
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