The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Peptide ELISA: Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.3 - 1.0 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
组织特异性Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.
通路Protein modification; protein ubiquitination.
序列相似性Contains 1 RING-type zinc finger.
翻译后修饰RING-type zinc finger-dependent and E2-dependent autoubiquitination.
细胞定位Cytoplasm > cytosol. The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.