概述

  • 产品名称Anti-ZNF312抗体
    参阅全部 ZNF312 一抗
  • 描述
    兔多克隆抗体to ZNF312
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: ASSASLETMV PPACPRAGAS PATSKTLAFS IERIMAKTSE PRAPFEPRPG, corresponding to amino acids 1-50 of Human ZNF312

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab49245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性ZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Fez antibody
    • FEZ family zinc finger protein 2 antibody
    • FEZF 2 antibody
    • FEZF2 antibody
    • FEZL antibody
    • Forebrain embryonic zinc finger like protein 2 antibody
    • TOF antibody
    • ZFP 312 antibody
    • ZFP312 antibody
    • Zinc finger FEZL antibody
    • Zinc finger protein 312 antibody
    • Zinc finger protein FEZ like antibody
    • ZNF 312 antibody
    see all

Anti-ZNF312 antibody 图像

  • Lane 0 : Marker
    Lane 1 : Anti-ZNF312 antibody (ab49245) at 2.5 µg/ml

    Lane 0 : As above
    Lane 1 : Jurkat cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    Lane 1 : HRP conjugated anti-rabbit IgG at 1/50000 dilution


    Predicted band size : 49 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ZNF312 antibody (ab49245) at 2.5 µg/ml

    Lane 1 : MW Ladder
    Lane 2 : Cell lysates prepared from human Jurkat cells


    Predicted band size : 49 kDa

Anti-ZNF312 antibody (ab49245)参考文献

ab49245 has not yet been referenced specifically in any publications.

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