The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
相关性A zinc finger is a protein domain that can bind to DNA. A zinc finger consists of two antiparallel ß sheets, and an alpha helix. The structure of each individual finger is highly conserved and consists of about 30 amino acid residues, constructed as a ßßalpha fold and held together by the zinc ion. The alpha helix occurs at the C terminal part of the finger, while the ß sheet occurs at the N terminal part.