概述

  • 产品名称Anti-ZNF3抗体
    参阅全部 ZNF3 一抗
  • 描述
    小鼠单克隆抗体to ZNF3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-110 of Human ZNF3 with proprietary tag.

  • 阳性对照
    • ZNF3 transfected 293T cell lysate. Recombinant tagged Human ZNF3 fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性A zinc finger is a protein domain that can bind to DNA. A zinc finger consists of two antiparallel ß sheets, and an alpha helix. The structure of each individual finger is highly conserved and consists of about 30 amino acid residues, constructed as a ßßalpha fold and held together by the zinc ion. The alpha helix occurs at the C terminal part of the finger, while the ß sheet occurs at the N terminal part.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • A8 51 antibody
    • C2 H2 type zinc finger protein antibody
    • FLJ20216 antibody
    • HF.12 antibody
    • HZF3.1 antibody
    • KOX25 antibody
    • PP838 antibody
    • Zfp113 antibody
    • Zinc finger protein 3 (A8 51) antibody
    • Zinc finger protein 3 antibody
    • Zinc finger protein HF.12 antibody
    • Zinc finger protein KOX25 antibody
    see all

Anti-ZNF3 antibody 图像

  • All lanes : Anti-ZNF3 antibody (ab77201) at 1 µg/ml

    Lane 1 : ZNF3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • Anti-ZNF3 antibody (ab77201) at 1 µg/ml + Recombinant tagged Human ZNF3 fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ZNF3 antibody (ab77201) at 1/1000 dilution

    Lane 1 : Cytoplasmic lysate prepared from human U2OS cells
    Lane 2 : Nuclear lysate prepared from human U2OS cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated anti-mouse IgG polyclonal at 1/4000 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Image courtesy of Dr Andrew Cobb by Abreview.

    See Abreview

Anti-ZNF3 antibody (ab77201)参考文献

ab77201 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (U2OS)
Loading amount 20 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

提交于 Apr 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"