Horse, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
Synthetic peptide, corresponding to a region between residue 879 and 929 of Human ZNF280D (NP_060131.2).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 109 kDa.
Use at 2-10 µg/mg of lysate.
May function as a transcription factor.
Contains 5 C2H2-type zinc fingers.
Information by UniProt
Western blot - Anti-ZNF280D antibody (ab114972)
All lanes : Anti-ZNF280D antibody (ab114972) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 109 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-ZNF280D antibody (ab114972)
Detection of ZNF280D in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114972 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"